Qualitative enzymatic detection of organophosphate and carbamate insecticides


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Nartop D., Hasanoğlu Özkan E. , Yetim N. K. , Sarı N.

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, cilt.55, ss.951-958, 2020 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 55
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/03601234.2020.1797425
  • Dergi Adı: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
  • Sayfa Sayıları: ss.951-958

Özet

In this study, new polymers containing amides (TrisPS-Ntaa, and TrisPS-Ntaa-Fc) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE.