Quantification and Penetration Properties of p-Hydroxybenzoic Acid Derivatives


CURRENT PHARMACEUTICAL ANALYSIS, vol.13, no.1, pp.18-25, 2017 (SCI-Expanded) identifier identifier


Parabens are widely used in many cosmetic formulations. Although there are some reports which correlate tumor growth with the use of parabens, available data on penetration through human skin is still not sufficient to fully understand their potential toxicity. The aim of this report is to investigate penetration properties of parabens through human skin. Methylparaben (MP), sodium methylparaben (NaMP), propylparaben (PP) and sodium propylparaben (NaPP) were selected as the most commonly used parabens in cosmetic formulations. In addition to UPLC method, a simple and rapid method based on dispersive liquid-liquid microextraction (DLLME) prior to capillary electrophoresis (CE) was also developed for determination of parabens. Penetration properties of parabens through full thickness of human skin were determined using Franz diffusion cell. For saturated solutions, the flux values for MP, PP, NaMP and NaPP were found to be 9.36 +/- 3.36, 4.09 +/- 1.03, 121 +/- 19 and 15.4 +/- 0.2 mu g/cm(2) h, respectively. Shampoo formulations gave a flux value of 0.545 +/- 0.271 mu g/cm(2) h for MP. The flux value of MP was found as 0.968 +/- 0.221 for hand cream, and MP and PP were 0.650 +/- 0.254 and 0.320 +/- 0.283 mu g/cm(2) h, respectively for sunscreen formulations. The flux value of MP was found as 0.677 +/- 0.273 mu g/cm(2) h for body lotion. The amount of penetrated parabens varied depending on the formulation. Hence, it was concluded that formulations should be investigated individually for paraben penetration and their possible toxic effect.