Poly(styrene-divinylbenzene) beads surface functionalized with di-block polymer grafting and multi-modal ligand attachment: performance of reversibly immobilized lipase in ester synthesis

BAYRAMOĞLU G., Karagöz B., Altintas B., Arica M. Y., Bicak N.

BIOPROCESS AND BIOSYSTEMS ENGINEERING, cilt.34, sa.6, ss.735-746, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 6
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s00449-011-0523-1
  • Dergi Adı: BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.735-746
  • Anahtar Kelimeler: Enzyme immobilization, Adsorption, Lipase, Kinetics, Ester synthesis, CANDIDA-RUGOSA LIPASE, TRANSFER RADICAL POLYMERIZATION, COVALENT IMMOBILIZATION, POLY(GMA-MMA) BEADS, REACTION PARAMETERS, MAGNETIC BEADS, ETHYL VALERATE, ADSORPTION, BRUSHES, MEMBRANES
  • Gazi Üniversitesi Adresli: Evet

Özet

Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface-initiated atom transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand attached beads were used for reversible immobilization of lipase. The influences of pH, ionic strength, and initial lipase concentration on the immobilization capacities of the beads have been investigated. Lipase adsorption capacity of the beads was about 78.1 mg/g beads at pH 6.0. The K (m) value for immobilized lipase was about 2.1-fold higher than that of free enzyme. The thermal, and storage stability of the immobilized lipase also was increased compared to the native lipase. It was observed that the same support enzyme could be repeatedly used for immobilization of lipase after regeneration without significant loss in adsorption capacity or enzyme activity. A lipase from Mucor miehei immobilized on styrene-divinylbenzene copolymer was used to catalyze the direct esterification of butyl alcohol and butyric acid.