A solid phase extraction (SPE) procedure based on Amberlite IRA 900 resin was developed for speciation and separation of inorganic arsenic species (III, V) and total As in water samples. The As species and total As in eluent solutions were determined by electrothermal atomic absorption spectrometry (ETAAS) using Ni chemical modifier with 1200 degrees C pyrolysis temperature. Experimental parameters such as pH value, sample volume, flow rate, volume and concentration of eluent solution for As(V) were optimised and 98.0 +/- 1.9% recovery was found at pH 4.0. Experimental adsorption capacity of the resin for As(V) was investigated and 229.9 mg g(-1) was found. Under optimised experimental conditions, instrumental parameters such as limit of detection (LOD) and limit of quantification (LOQ) found were 0.126 and 0.420 mu g L-1, respectively. Interference effects of coexisting ions in the sample matrix on the recovery of As(V) were investigated. Concentration of As(III) was obtained by subtracting As(V) concentration found at pH 4.0 from total As(III + V) found at pH 8.0. The accuracy of the method proposed by using the resin was tested for analysing As species in a waste water standard reference material (SRM, CWW-TM-D) and spiked real water samples with recovery above 95%. The method proposed was also applied to the determinations of As species and total As in underground hot waters and tap water with relative error below 3%.