The effects of thymoquinone and genistein treatment on telomerase activity, apoptosis, angiogenesis, and survival in thyroid cancer cell lines

Ozturk S. A., Alp E., Yar Sağlam A. S., Konaç E., Menevse E. S.

JOURNAL OF CANCER RESEARCH AND THERAPEUTICS, vol.14, pp.328-334, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14
  • Publication Date: 2018
  • Doi Number: 10.4103/0973-1482.202886
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.328-334
  • Keywords: Anaplastic thyroid cancer, follicular thyroid cancer, genistein, thymoquinone, thyroid cancer, FACTOR-KAPPA-B, PROSTATE-CANCER, STEM-CELLS, GROWTH, SUPPRESSION, CARCINOMA, PATHWAY, CARCINOGENESIS, MECHANISMS, ARREST
  • Gazi University Affiliated: Yes


Context: Thyroid cancers (TCs) are the most common endocrine malignancies. There were two problems with the current cancer chemotherapy: the ineffectiveness of treatment due to resistance to cancer cell, and the toxic effect on normal cells.
Aims: This study was aimed to determine the effects of thymoquinone (TQ) and genistein (Gen) phytotherapeutics on telomerase activity, angiogenesis, and apoptosis in follicular and anaplastic thyroid cancer cells (TCCs).
Materials and Methods: Cell viability, caspase‑3 (CASP‑3) activity, and messenger RNA (mRNA) expression levels of human telomerase reverse transcriptase (hTERT), phosphatase and tensin homolog (PTEN), nuclear factor‑kappa B (NF‑kB), cyclin-dependent kinase inhibitor 1 (p21), and vascular endothelial growth factor‑A (VEGF‑A) genes were analyzed.
Results: It was found that TQ and Gen treatment on TCCs caused a statistically significant decrease of cell viability, and mRNA expression levels of hTERT, VEGF‑A, and NF‑kB genes, but a statistically significant increase of PTEN and p21 mRNA expression levels. In addition, TQ and Gen treatment also caused a statistically significant increase active CASP‑3 protein level in TCCs. Moreover, our results demonstrated that, when compared with follicular TCCs, anaplastic TCCs were more sensitive to the treatment of TQ and Gen.