Polyethyleneimine-grafted poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization

Arica M., BAYRAMOĞLU G.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.20, sa.1, ss.73-77, 2004 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 1
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.bej.2004.04.008
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.73-77
  • Gazi Üniversitesi Adresli: Hayır

Özet

Glucose oxidase (GOD) was immobilized onto polyethyleneimide (PEI) grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) (poly(HEMA-co-GMA)) membranes. The maximum GOD immobilization capacities of the poly(HEMA-co-GMA-4)-PEI membrane were 84 mug/cm(2). The activities of GOD-immobilized onto poly(HEMA-co-GMA-1-5)-PEI membranes were in the range 7.6-14.5 U/cm(2). The storage conditions for the immobilized enzyme preparations were studied and the immobilized enzymes had a long-storage stability (only 7 and 24% activity decrease in 3 months under wet and dry storage conditions, respectively). (C) 2004 Elsevier B.V. All rights reserved.