A New Laccase-Based Biosensor for Epinephrine Determination
GAZI UNIVERSITY JOURNAL OF SCIENCE, cilt.28, sa.1, ss.1-9, 2015 (ESCI, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 1
- Basım Tarihi: 2015
- Dergi Adı: GAZI UNIVERSITY JOURNAL OF SCIENCE
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
- Sayfa Sayıları: ss.1-9
- Gazi Üniversitesi Adresli: Evet
Özet
In this paper, a novel amperometric epinephrine (adrenaline) biosensor with immobilization of laccase on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been a complished. Laccase enzyme were immobilized on PPy-PVS film by cross-linking with glutaraldehyde. Determination of epinephrine (EP) was carried out by the reduction of enzymatically produced epinephrinequinone at - 0.220 V vs. Ag/AgCl. The effects of pH and temperature were investigated. There are two linear parts in the region between 0.1 - 1.0 mu M and 1.0 - 10.0 mu M. The storage stability and operation stability of the enzyme electrode were also studied. Interference effects were investigated on response of the biosensor.