Purpose: The tongue is a complex muscular organ constituted of several intrinsic and extrinsic muscles, innervated by several cranial nerves. Methods: In this study, the nerves and their branches in the tongue were demonstrated by Sihler's stain technique. Results: The distributions of the hypoglossal nerve in the muscles of the tongue were visualized. The sensitive fiber contribution of the glossopharyngeal nerve to the posterior one-third of the tongue was followed. In addition, the course of the lingual nerve and its branchings in the corpus towards the tip were demonstrated. Conclusion: We think that this overlooked stain technique should be considered when studying the complex innervations of structures, especially in re-innervation experiments.