Serum INHB levels and ACE gene I/D polymorphism with increased risk for unexplained infertility


TURAN T., Pekel A., Duvan Z. C., GÖNENÇ A.

JOURNAL OF BIOCHEMISTRY, vol.170, no.2, pp.245-253, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 170 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.1093/jb/mvab036
  • Journal Name: JOURNAL OF BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.245-253
  • Keywords: angiotensin converting enzyme (ACE), anti-Mullerian hormone (AMH), inhibin-B (INHB), polymorphism, unexplained infertility (UI), ANGIOTENSIN-CONVERTING ENZYME, POLYCYSTIC OVARIAN SYNDROME, RECURRENT PREGNANCY LOSS, ANTI-MULLERIAN HORMONE, INHIBIN-B, INSERTION/DELETION POLYMORPHISM, FOLLICULAR-FLUID, WOMEN, ASSOCIATION, PREVALENCE
  • Gazi University Affiliated: Yes

Abstract

Angiotensin converting enzyme (ACE) has a significant role in the angiogenesis of ovarian endothelium and the resumption of meiosis and folicular growth. However, there is no any study concerning ACE polymorphism and unexplained infertility (UI). The main aim of this study is that both identify ACE polymorphism and measure the serum ACE, anti-Mullerian hormone (AMH) and inhibin-B (INHB) levels in UI patients and controls in Turkish population. Forty-seven UI patients and 41 controls were involved in this study. To determine the ACE polymorphisms, DNA isolation and PCR were performed. Then, serum ACE, AMH and INHB levels were measured spectrophotometrically. Patients with UI had significantly higher serum INHB levels compared with controls (P< 0.05). Serum ACE levels were decreased, compared to controls; however, the decrease was not significant. Serum AMH levels did not significantly differ from controls. When the relationship was analysed between ACE insertion/deletion (I/D) polymorphism and infertility risk, and ID genotype was chosen as reference, it was found to be 2.33 times more risk of UI than the women have DD genotype IDD versus ID: odds ratio = 2.33, 95% confidence interval (0.88-6.19); P = 0.086]. This finding indicates that DD genotype may be high risk for UI. Further studies are warranted to confirm this finding, especially with a larger population.