Placental activator and inhibitor miRNAs efficiency on abortion development; an epigenetic alteration on true fetal tissues-poster number is P1110

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Kazancıoğlu E., Tuğ E., Ergün M. A., Kazan H. H., Yirmibeş Karaoğuz M.

14th European Cytogenomics Conference, Montpellier, Fransa, 1 - 04 Temmuz 2023, cilt.52, ss.81-82

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 52
  • Basıldığı Şehir: Montpellier
  • Basıldığı Ülke: Fransa
  • Sayfa Sayıları: ss.81-82
  • Gazi Üniversitesi Adresli: Evet

Özet

Background: Small non-coding micro ribonucleic acids, namely miRNAs or MIRs are associated with defective placentation due to their role on the proliferation of the trophoblasts. Relationship between the abortion development and placental defects lead us to search the placental activator (MIR378a-5p, MIR376c, and MIR195) and inhibitor (MIR34a and MIR210) miRNAs expression levels on the accurate pregnancy loss tissues. To this end, XX abortion materials are omitted from the study to exclude the maternal component.

Methods: Total RNA isolation was performed with TRIzol™ reagent directly from frozen trophoblastic tissues belonging to 42 disomic XY, 43 aneuploidic, and 15 triploidic pregnancy loss tissues. All materials were searched for the miRNA expression levels after the mature miRNA levels obtained by reverse transcription via Quantitative Real-Time Polymerase Chain Reaction. This study was supported by the Scientific Research Fund of Gazi University (Grant TTU-2021-6959) and the preliminary results were presented at the 15th National Congress of Medical Genetics, held on November 2022, at Mugla-Turkey.
Results: The expression of placental inhibitor MIR34a on trisomic trophoblastic tissues was in a high level when compared to the disomic ones (p=0.0324). An increase in MIR195 (p=0.0484) and MIR34a (p=0.0346) expression levels was observed in numerically abnormal trophoblastic tissues with advanced maternal age group when compared with the disomic ones (with all ages).
Conclusions: It seems likely that the oocyte aging process, which increases with advanced maternal age, contributes to the development of spontaneous abortion with the occurence of high expression levels of MIR195 and MIR34a. In sum, this study has value to demonstrate the importance of miRNA expression levels in defective placentation and abort pathophysiology as an epigenetic approach, with the necessity of more research on this topic