Surface modification and covalent coupling of concanavalin a onto poly(EGDMA/HEMA) microbeads for cell affinity applications


Ayhan F.

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, cilt.18, sa.4, ss.297-310, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Konu: 4
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1177/088391103037007
  • Dergi Adı: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
  • Sayfa Sayıları: ss.297-310

Özet

The surfaces of nonporous and porous Poly(EGDMA/HEMA) microbeads were modified by the reaction of sodium periodate, hexamethylenediamine and glutaraldehyde. Covalent immobilization of Concanavalin A (Con A) was optimized for maximum loading at various Con A concentrations, pH, temperatures, and ionic strengths. The optimal Con A immobilized concentration was determined as 0.37 and 0.75 mg/g microbeads for the nonporous and porous microbeads, respectively. Covalent coupling was achieved at 37degreesC, with 0.5 mg/mL of Con A at pH 7.4 and ionic strength of 0.01. The specific activity of Con A immobilized microbeads to carbohydrate structure was tested for affinity for myeloma HeLa cells. The data indicates that Con A may play a role in myeloma cell adhesion.