Enhanced ethyl butyrate production using immobilized lipase


Ates S., Turk B., BAYRAKTAR E., GÜVENÇ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.41, sa.5, ss.339-343, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Konu: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/10731199.2012.743902
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.339-343

Özet

In this study, the production of ethyl butyrate was investigated by using immobilized lipase enzyme in shake flasks. In order to determine optimum conditions for the production, response surface methodology was used. The model indicated the optimum conditions for maximum conversion (9.1%) at the 0.31 M substrate concentration, acid- alcohol molar ratio of 0.49, immobilized enzyme 25% (w/v) at 35 degrees C, for 3 hours which were in good agreement with the experimental value. At the end of the 55 hours conversion was obtained as 61.3%. When Na2HPO4 was used in reaction medium conversion increased to 90.3% for 55 hours.