Indigofera zollingeriana is an important protein rich forage and dye plant species that grows widely in Indonesia. It is difficult to propagate the plant through seeds due to high dormancy. Therefore, there is need to develop both in vitro and ex vitro propagation techniques of I. zollingeriana. This study aimed to develop a protocol for multiple shoot regeneration of the plant under in vitro conditions using different combinations of BAP+0.01 mg/L NAA. The results showed that both epigeal and cotyledon node explants have high potential to regenerate with maximum number of regenerated shoots noted on the epigeal and the cotyledon node explants using MS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0 mg/L BAP + 0.01 mg/L of NAA in the same order. The shoots regenerated on all combinations of BAP+NAA were rooted using 0.5 mg/L IBA that promoted rooting in effective manner. The rooted plants were easily acclimatized to external conditions in an environmental chamber. The results suggest that I. zollengriana plant species is no more difficult to regenerate under in vitro conditions. This research will aid in future studies related to breeding, propagation, biosynthesis and extraction of active compounds.