Çubuk Benzeri TiO2, Pedot Kaplı Çubuk Benzeri TiO2 ve Pedot Nanopartiküllerinin in vitro Genotoksik Etkisi

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Duran İ. F., Ünal F., Yüzbaşıoğlu D.

IV. International Ankara Conference on Scientific Research , Ankara, Türkiye, 10 - 11 Nisan 2021, ss.83-84

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.83-84
  • Gazi Üniversitesi Adresli: Evet

Özet

Titanium dioxide (TiO2) nanoparticles (NPs) are widely used in medical, pharmaceuticals, paints, plastics, paper, food, and cosmetics. However, several studies have shown that NPs may induce toxic effects such as inflammation and oxidative stress, even cytotoxic and genotoxic effects. Therefore, we assessed the in vitro genotoxic effects of rod-like TiO2 (R-TiO2-NPs) and pedot [poly(3,4-ethylenedioxythiophene)] coated rod-like TiO2 nanoparticles (Pd/Rd-TiO2-NPs) at 50-500 µg/mL and pedot nanoparticles (Pd-NPs) at 5-50 µg/mL concentrations on human lymphocytes using chromosome aberration (CA) test. Seven different types of structural (chromatid and chromosome break, sister chromatid union, dicentric chromosome, sister chromatid exchange, fragment and ring) and one type of numerical (polyploidy) chromosome aberrations (CAs) were evident in human lymphocytes. All the particles increased the frequency of CAs and CA/Cell at both exposure periods. R-TiO2-NPs, in the 24 h treatment, significantly increased the frequency of CAs and CA/cell in two lowest concentrations (50 and 100 μg/mL) compared to negative control. In the 48 h treatment, both the CA% and CA/Cell significantly raised at all the concentrations used. P/R-TiO2 NPs caused significant increase in the frequency of CAs and CA/Cell at 250 and 500 μg/mL, at 24 and 48 h exposure, respectively. 50 μg/mL also significantly increased CA/Cell at 48 h. In case of Pd-NPs, concentrations applied were lower (5, 10, 25 ve 50 μg/mL) than the other two NPs because 100, 200, and 500 μg/mL showed cytotoxic effect. Significant increase in the same parameters were determined at the two highest concentrations (25 and 50 μg/mL) in the 24 h treatment. 5 μg/mL significantly increased the frequency of CAs as well. None of the increasing was significant in the 48 h treatment. These results revealed that R-TiO2-NPs have strong clastogenic action compared to Pd/R-TiO2 and pedot NPs alone, the last two NPs showed similar and weak clastogenic effect in human lymphocytes. Therefore, TiO2 NPs should be produced and used with great attention till more investigations reveal safety.