EFFECT OF NEW SULFONYL HYDRAZONE COMPOUNDS AGAINST CANDIDA BIOFILMS

Aydın M., Öztürk A., Duran T., Özdemir Özmen Ü., Sumlu E.

MESMAP – 7, İzmir, Türkiye, 18 - 20 Kasım 2021, ss.57

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.57
  • Gazi Üniversitesi Adresli: Evet

Özet


Abstract

Objectives: Candidiasis is the most frequently encountered fungal infection, with ranges from mild superficial infections to disseminated candidiasis. Most manifestations of Candidiasis are

associated with biofilm formation. Candida biofilms contributes to therapeutic failure due to resistance to antimicrobial agents and causes an increase in mortality rates. For these reasons,

finding new drugs and antimicrobial agents are urgently needed. Sulfonyl hydrazones derived from sulfonamide, are well-known for its pharmacological effects, such as antifungal,

antibacterial potential activity. This study aimed to investigate the anti-biofilm activity of the new sulfonyl hydrazone compounds (Anaf-Bsh, Anaf-Esh, Anaf-Psh) against Candida species.

Methods: Candida biofilms was carried out in a 96-well flat-bottom plate. Biofilm biomass was measured using Crystal violet staining method. Forty-two isolates tested were classified

according to their ability to produce biofilms and 10 isolates that have strong biofilms producer were selected. The inhibitory effects of each compound (0.5–256 μg/ml) on Candida biofilms

were measured MTT assay. The total RNA was isolated from the control and treated biofilm cells using TRIzol reagent. Synthesis of cDNA from total RNA was carried out using

Transcriptor High Fidelity cDNA Synthesis kit. Expression analysis of biofilm related key genes (ALS3, HWP1, UME6, SAP5, ECE1) was investigated with the RT-qPCR method. Fold

changes were calculated by the 2-ΔΔCT method. The statistical analysis was performed using one-way ANOVA test. Results: In RT-qPCR experiments, changes in the expression levels of

biofilm-related key genes have been observed after sulfonyl hydrazone compounds.

Conclusions: These results indicate that sulfonyl hydrazone compounds may have therapeutic potential in the treatment and prevention of biofilm-associated Candida infections.

Key Words: Biofilm, biofilm related genes, Candida, sulfonyl hydrazone compounds.