Torsion/Detorsion of the testis does not modify responses to nitric oxide in rat isolated penile bulb


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Ozkan M. H. , Vural I. M. , Moralioglu S., Uma S., Sarioglu Y.

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, vol.101, no.2, pp.117-120, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 101 Issue: 2
  • Publication Date: 2007
  • Doi Number: 10.1111/j.1742-7843.2007.00087.x
  • Title of Journal : BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
  • Page Numbers: pp.117-120

Abstract

Ischaemia-reperfusion damage induced by torsion/detorsion of the testicles may be a causative factor leading to erectile dysfunction through oxidative stress-dependent changes in the responses of the penile bulb, an erectile tissue of the penis. We aimed at investigating the effects of unilateral testicular torsion/detorsion (2 or 24 hr) treatment on relaxations induced by electrical field stimulation and sodium nitroprusside in rat isolated penile bulb. Male Sprague-Dawley rats used in the study were divided into two groups. The treatment group was subjected to unilateral torsion followed by detorsion for 2 or 24 hr, while the control group underwent only sham operation. For in vitro organ bath experiments, penile bulbs were isolated and responses to relaxant agents and electrical field stimulation (70 V, 1 msec., 0.5-8 Hz, 5 sec.) were recorded on a computer-based data acquisition system via a force displacement transducer. In tissues precontracted with phenylephrine (3 x 10(-6)M), relaxations induced by electrical field stimulation were not significantly different before and after 2 or 24 hr of detorsion. Similarly sodium nitroprusside- (10(-8)-3 x 10(-6)M) and papaverine-induced (10(-7)-10(-4)M) relaxations were also found unchanged in the detorsion group compared to control. In conclusion, spermatic cord torsion did not lead to impairment in nitric oxide-mediated relaxant responses of the rat isolated penile bulb.