Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms


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Nartop D., Demirel B., Güleç M., Hasanoğlu Özkan E., Yetim N. K. , Sarı N., ...More

Journal of Biochemical and Molecular Toxicology, vol.34, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34
  • Publication Date: 2020
  • Doi Number: 10.1002/jbt.22432
  • Journal Name: Journal of Biochemical and Molecular Toxicology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE
  • Keywords: antimicrobial property, antimutagenic effect, glucose oxidase, Polymeric microsphere, Pt4+-azomethine, BOUND SCHIFF-BASES, GLUCOSE-OXIDASE, COVALENT IMMOBILIZATION, COMPLEXES SYNTHESIS, SODIUM-AZIDE, GENOTOXICITY, DAMAGE
  • Gazi University Affiliated: Yes

Abstract

New polymeric microspheres containing azomethine (1a‐1c and 2a‐2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT‐IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis‐Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (Vmax) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well‐diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests.