Akademik Veri Yönetim Sistemi
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.75, sa.4, 2025 (SCI-Expanded, Scopus)
This study aimed to evaluate the effect of metformin on the regulation of the ovarian cycle through serum antioxidant activities in female rats fructose-induced metabolic syndrome model. Thirty-four, immature female Wistar rats (21 days-old) were randomly divided into five groups (n:4-8/group) control, carboxymethylcellulose (CMC), fructose, metformin, and fructose+metformin groups. The metabolic syndrome model was induced by fructose solution (20% w/v) for 15 weeks. Metformin was administrated by oral gavage in CMC solution for the last final 6 weeks. Vaginal cytology was evaluated before and at the 9th and 15th weeks of the experiment. Total antioxidant status (TAS), total oxidant status (TOS), and paraoxonase (PON)-1 levels were assessed in serum samples. The statistical analysis was performed using a one-way ANOVA test. Results are expressed as the mean +/- standard error of the mean (SEM). Metformin restored the regularity of the estrous cycle, which was disturbed by a high fructose diet. Moreover, dietary high-fructose markedly increased serum TOS and OSI values, but significantly decreased serum TAS and PON-1 levels than the other groups (p < 0.05). Metformin, through its antioxidant activity, may have had a beneficial effect on the impaired ovarian cycle associated with oxidative stress induced by high fructose diet.