DNA repair enzyme polymorphisms and oxidative stress in a Turkish population with gastric carcinoma


ENGİN A. B. , KARAHALİL B. , Engin A., Karakaya A. E.

MOLECULAR BIOLOGY REPORTS, vol.38, no.8, pp.5379-5386, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 8
  • Publication Date: 2011
  • Doi Number: 10.1007/s11033-011-0690-9
  • Title of Journal : MOLECULAR BIOLOGY REPORTS
  • Page Numbers: pp.5379-5386
  • Keywords: Gastric cancer, OGG1 Ser326Cys polymorphism, XPC Lys939Gln polymorphism, XPD Lys751Gln polymorphism, Helicobacter pylori, Oxidative stress, NITRIC-OXIDE SYNTHASE, HELICOBACTER-PYLORI, GENETIC POLYMORPHISMS, CANCER RISK, HOGG1 GENE, DAMAGE, XPD, ADENOCARCINOMA, EXPRESSION, 8-HYDROXYGUANINE

Abstract

Although the developmental stages of gastric carcinoma are still not clear, the constantly generated reactive oxygen and nitrogen species (ROS/RNS) may contribute to the process of carcinogenesis by interacting with DNA. 8-oxoguanine DNA glycosylase-1 (OGG1) is an enzyme involved in base excision repair of 8-oxoguanine that is one of the premutagenic lesions generated by ROS in DNA. The bulky adducts, are recognized and repaired by nucleotid excision repair (NER) enzymes, including xeroderma pigmentosum C and D (XPC, XPD). Eligible 106 gastric cancer patients and 116 cancer-free individuals constituted the study and control groups, respectively. Association between OGG1 Ser326Cys, XPC Lys939Gln, XPD Lys751Gln polymorphisms and the susceptibility tho cancer and the oxidative stress status were evaluated. DNA was extracted from peripheral blood cells and genotypes were determined by using PCR-RFLP. Serum nitric oxide, albumin concentrations, total antioxidant status and Helicobacter pylori IgG were determined. Serum albumin and nitric oxide of cancer patients were lower than that of the controls (P < 0.05). None of the evaluated polymorphisms or Helicobacter pylori IgG seropositivity associated with increased risk of gastric cancer, despite of the increased oxidative stress in cancer patients.