Polystyrene Attached Pt(IV)-Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme

SARI N., Antepli E., NARTOP D., Yetim N. K.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, cilt.13, sa.9, ss.11870-11880, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 9
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3390/ijms130911870
  • Dergi Adı: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.11870-11880
  • Anahtar Kelimeler: Pt(IV)-azomethine, Polystyrene, glucose oxidase enzyme, two optimum pH, SCHIFF-BASES, ASPERGILLUS-NIGER, SUPPORTS, MECHANISM
  • Gazi Üniversitesi Adresli: Evet

Özet

Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 degrees C.