Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.27, no.2, pp.313-320, 2023 (Peer-Reviewed Journal)
Abstract: Ferri and ferro coordination polymers in sphere structure were
synthesized. Scanning Electron Microscopy (SEM) Energy Dispersive X-Ray
Spectroscopy (EDX), Gel Permeation Chromatography (GPC), elemental analysis,
and Fourier Transform Infrared Spectroscopy (FT-IR) were performed for chemical
and structural characterization of the coordination polymers. Glucose oxidase
(GOD) enzyme immobilized to compare of kinetic parameters deal with glucano-1,5
lacton formation. Analyses results illustrate that structures coordination of ions Fe2+
and Fe3+ are different to the same support. It was seen that 2 mol of Fe2+ ion ((PS-N-
([Fe(CN)4L]K3)2) was bound per unit structure while 1 mol of Fe3+ ion (PS-N-
([Fe(CN)2L]K)) is attaching. Km values of were found as 15.32 and 10.93 for (PS-NFe2+)-GOD and (PS-N-Fe3+)-GOD, respectively. Km value for (PS-N-Fe3+)-GOD was
found to be 0.5 times higher, possible reason of such a case is the larger reduction
potential of Fe3+. As the charge on the coordination structure increased, the
enzyme's affinity for the substrate increased. After 20 repeated measurements, GOD
immobilized on (PS-N-Fe3+) polymer retained 45.47% activity, while GOD
immobilized on (PS-N-Fe2+) polymer retained 57.86% activity.