In vitro neuroprotective effects of the leaf and fruit extracts of Juglans regia L. (walnut) through enzymes linked to Alzheimer's disease and antioxidant activity


ERDOĞAN ORHAN İ. , SÜNTAR İ. , AKKOL E.

INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, vol.62, no.8, pp.781-786, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 62 Issue: 8
  • Publication Date: 2011
  • Doi Number: 10.3109/09637486.2011.585964
  • Title of Journal : INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
  • Page Numbers: pp.781-786
  • Keywords: Juglans regia, walnut, cholinesterase inhibition, antioxidant activity, PHENOLIC-COMPOUNDS, TURKEY

Abstract

Several extracts of the leaves and fruits of Juglans regia L. were assessed for their neuroprotective effects through antioxidant and anti-cholinesterase methods. Anticholinesterase activity was determined against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), the enzymes vital for Alzheimer's disease, at 50, 100 and 200 mu g ml(-1). Antioxidant activity was tested using radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylenediamine (DMPD), superoxide (SO), nitric oxide (NO) and hydrogen peroxide (H2O2) radicals, as well as ferric ion-chelating capacity, ferric-and phosphomolybdenum-reducing antioxidant power at 500, 1000 and 2000 mu g ml(-1). Total phenol and flavonoid quantification of the extracts was calculated. The extracts scavenged DPPH radical in varying degrees; however, they did not scavenge DPMD and H2O2. Only the dichloromethane and water extracts were able to quench SO (10.09 +/- 1.38%) and NO (24.09 +/- 2.19%) radicals, respectively, at low level. The extracts showed either low or no BChE inhibition and no AChE inhibition.