Investigation of acute toxicity of (2,4-dichlorophen oxy) acetic acid (2,4-D) herbicide on crayfish (Astacus leptodactylus Esch 1823)

Benli A. Ç. , Sarikaya R. , Sepici-Dincel A. , Selvi M. , Sahin D., Erkoc F.

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, cilt.88, sa.3, ss.296-299, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 88 Konu: 3
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.pestbp.2007.01.004
  • Sayfa Sayıları: ss.296-299


The acute 96 h LC50 of (2,4-dichlorophenoxy)acetic acid (2,4-D), a widely used agricultural herbicide, was determined on crayfish (Astacus leptodactylus Esch. 1823). Crayfish of 23.5 1.49 g mean weight and 9.6 +/- 0.21 cm mean length were selected for the bioassay experiments. The experiments were repeated three times, in 10 L tap water. The data obtained were statistically evaluated by the use of the E.P.A computer program based on Finney's probit analysis method and the 96 h LC50 value for crayfish was calculated to be 32.6 mg/L in a static bioassay test system. 95% lower and upper confidence limits for the LC50 were 15.10-327.16. In conclusion, 2,4-D is highly toxic to crayfish, a non-target organism in the ecosystem. Water temperature was 23 +/- 1 degrees C. Behavioral changes of crayfish were recorded for all herbicide concentrations. (c) 2007 Elsevier Inc. All rights reserved.