Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro

Creative Commons License

Eroglu S., PANDIR D., Uzun F. G., Bas H.

BIOLOGICAL RESEARCH, cilt.46, sa.1, ss.33-38, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.4067/s0716-97602013000100005
  • Dergi Adı: BIOLOGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.33-38
  • Anahtar Kelimeler: antioxidant enzyme, dichlorvos, oxidative stress, vitamin, malondialdehyde, LIPID-PEROXIDATION, ANTIOXIDANT ENZYMES, CHLORPYRIFOS-ETHYL, ALPHA-TOCOPHEROL, MALE RATS, DICHLORVOS, TOXICITY, EXPOSURE, DAMAGE, VIVO
  • Gazi Üniversitesi Adresli: Evet

Özet

Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 mu M) or DDVP + vitamin C (VC; 10 mu M) or vitamin. E (VE; 30 mu M), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical differences between all groups for 1 mu M concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P < 0.05). Groups treated with vitamins and DDVP showed protective effects of vitamins against DDVP-induced changes in antioxidant enzyme activity and lipid peroxidation (LPO) (10 mu M). At 100 mu M concentration of DDVP vitamins had no effect on DDVP-induced toxicity. The results show that administration of DDVP resulted in the induction of erythrocyte LPO and alterations in antioxidant enzyme activities, suggesting that reactive oxygen species (ROS) may be involved in the toxic effects of DDVP. Also the data show that the plasma level of VC and VE may ameliorate OP-induced oxidative stress by decreasing LPO in erythrocytes at certain doses of OP pesicides.