SYNTHESIS OF NEW 6-[4-(2-FLUOROPHENYLPIPERAZINE-1-YL)]-3(2H)-PYRIDAZINONE-2-ACETHYL-2-(SUBSTITUTEDBENZAL)HYDRAZONE DERIVATIVES AND EVULATION OF THEIR CYTOTOXIC EFFECTS IN LIVER AND COLON CANCER CELL LINES

ÖZDEMİR Z., BAŞAK TÜRKMEN N., Ayhan I., ÇİFTÇİ O., UYSAL M.

PHARMACEUTICAL CHEMISTRY JOURNAL, vol.52, no.11, pp.923-929, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 11
  • Publication Date: 2019
  • Doi Number: 10.1007/s11094-019-01927-y
  • Journal Name: PHARMACEUTICAL CHEMISTRY JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.923-929
  • Keywords: pyridazinone, cytotoxicity, liver cancer, colon cancer, BIOLOGICAL EVALUATION, ACETYLCHOLINESTERASE, PYRIDAZINONE, 3(2H)-PYRIDAZINONE
  • Gazi University Affiliated: Yes

Abstract

In this study, seven new 3(2H)-pyridazinone derivatives expected to show cytotoxic activity in liver and colon cancer cell lines were synthesized. Their structures were confirmed by the IR, H-1-NMR, C-13-NMR spectra and elementary analyses. Compunds V-1-V-7 were tested on HEP3B (liver cancer) and HTC116 (colon cancer) cell lines for cytotoxicity by using MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] proliferation assay. Human fibroblast cells were used as safety control in these tests. 6-[4-(2-Fluorophenyl)piperazine-1-yl]-3(2H)-pyridazinone-2-acetyl-2-(2-chlorobenzal) hydrazone (compound V-3) was the most active agent with respect to HEP3B and HTC116 cell lines.