In this study, short-time Fourier transform (STFT) and wavelet transform (WT) were used for spectral analysis of ophthalmic arterial Doppler signals. Using these spectral analysis methods, the variations in the shape of the Doppler spectra as a function of time were presented in the form of sonograms in order to obtain medical information. These sonograms were then used to compare the applied methods in terms of their frequency resolution and the effects in determination of spectral broadening in the presence of ophthalmic artery stenosis. A qualitative improvement in the appearance of the sonograms obtained using the WT over the STFT was noticeable. Despite the qualitative improvement in the individual sonograms, no quantitative advantage in using the WT over the STFT for the determination of spectral broadening index was obtained due to the poorer variance of the wavelet transform-based spectral broadening index and the additional computational requirements of the wavelet transform. (C) 2003 Elsevier Ltd. All rights reserved.