A novel matrix for the immobilization of acetylcholinesterase


Sahin F., DEMİREL G. , Tumturk H.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.37, no.3, pp.148-153, 2005 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 3
  • Publication Date: 2005
  • Doi Number: 10.1016/j.ijbiomac.2005.10.003
  • Title of Journal : INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Page Numbers: pp.148-153
  • Keywords: immobilization, acetylcholinesterase, kappa-carrageenan, alginate, polymer blends, TETRATHIAFULVALENE TETRACYANOQUINODIMETHANE, CHOLINESTERASE IMMOBILIZATION, ELECTRODES, SENSORS

Abstract

In this study, a new matrix for immobilization of acetylcholinesterase was investigated by using alginate and kappa-carrageenan. The effects of pH, temperature, storage and thermal stability on the free and immobilized acetylcholinesterase activity were examined. Maximum reaction rate (V-max) and Michael is-Menten constant (K-m) was also investigated for free and immobilized enzymes. For free and immobilized enzymes into Ca-alginate and alginate/kappa-carrageenan polymer blends, optimum pH and temperature was found to be 7 and 30 degrees C, respectively. For free enzyme, maximum reaction rate (V-max) and Michaelis-Menten constant (K-m) values were found to be 6.35 mM and 50 mM min(-1), respectively, the same values for immobilized enzymes were determined as 8.68, 12.7mM and 39.7, 52.9 mM min(-1), respectively. Storage and thermal stability of acetylcholinesterase was increased by as a result of immobilization. (c) 2005 Elsevier B.V. All rights reserved.