A novel and highly sensitive electrochemical sensor based on a high-quality modified graphene electrode for the determination of hydrochlorothiazide in pharmaceutical formulations and human plasma

ER E., ÇELİKKAN H., Aksu M. L., ERK N.

ANALYTICAL METHODS, vol.7, no.21, pp.9254-9260, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 21
  • Publication Date: 2015
  • Doi Number: 10.1039/c5ay01980j
  • Journal Name: ANALYTICAL METHODS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.9254-9260
  • Gazi University Affiliated: Yes

Abstract

This study is concerned with the development of a practical, efficient and highly sensitive electrochemical sensor for the determination of hydrochlorothiazide (HCT) in pharmaceutical dosage forms or clinical samples using a high-quality modified graphene electrode (H-GRE) which has recently emerged as a very promising material for sensor applications. High-quality graphene (H-GR) was synthesized efficiently by a single-step reduction method from graphene oxide (GO). It was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and transmission electron spectroscopy (TEM). The electrochemical behavior of HCT was investigated using adsorptive stripping differential pulse voltammetry on the H-GRE. The H-GRE showed excellent electrochemical activity for the oxidation of HCT. Under the optimized experimental conditions, the linear response range of HCT was found to be 0.1-48.0 mu M with a detection limit (based on S/N-3) of 19 nM. This improved electrode may provide a promising alternative sensor for the determination of HCT in pharmaceutical formulations and clinical samples.