Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis


Eroglu Z. T., Kurtis B., Altug H. A., Sahin S., TÜTER G., BARIŞ E.

JOURNAL OF APPLIED ORAL SCIENCE, cilt.27, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1590/1678-7757-2018-0015
  • Dergi Adı: JOURNAL OF APPLIED ORAL SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Ozone, Transforming growth factor beta (TNF-beta), Vascular endothelial growth factor (VEGF), DONOR SITE WOUNDS, OZONATED OIL, OZONE, OXYGEN, MODEL
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-beta) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-beta expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.