Synthesis and spectroscopic characterization of superparamagnetic beads of copolymers of methacrylic acid, methyl. methacrylate and ethylene glycol dimethacrylate and their application to protein separation

BAYRAMOĞLU G., Arica M. Y.

POLYMER INTERNATIONAL, cilt.57, sa.1, ss.70-76, 2008 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57 Sayı: 1
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1002/pi.2313
  • Dergi Adı: POLYMER INTERNATIONAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.70-76
  • Gazi Üniversitesi Adresli: Hayır

Özet

Novel magnetic beads were prepared from methacrylic acid, methyl methacrylate and ethylene glycol dimethacrylate in the presence of ferric ions via suspension polymerization. After polymerization, thermal treatment of the Fe(III)-ion containing beads with Fe(II) ions under alkaline condition resulted encapsulation of Fe3O4 crystals within the polymer matrix. The magnetic beads were characterized by surface area measurement, swelling tests, electron spin resonance (ESR), differential scanning calorimetry (DSC), differential thermal analysis (DTA) and scanning electron microscopy (SEM). To evaluate the magnetic properties of the composite beads, Mossbauer spectra measurements were carried out. ESR and Mossbauer spectroscopy data revealed that the beads were highly superparamagnetic. The DSC data of the magnetic beads also showed an enhanced glass transition temperature owing to the presence of the Fe3O4 crystals within the polymer network. In addition, the presence of functional carboxyl groups on the surface permits further modifications in future applications. The magnetic beads were used for separation of human serum albumin and cytochrome c from aqueous solution in a batch system. (c) 2007 Society of Chemical Industry.