© 2022 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.Endocrine-disrupting chemicals like bisphenol A (BPA) and its analogs have negative effects on human health. This research aims to determine the cytotoxic effects of BPA and its four different analogs bisphenol S (BPS), bisphenol F (BPF), bisphenol Z (BPZ), bisphenol AF (BPAF) on both cancer and healthy cell lines simultaneously by performing an MTT test. In this study, human breast cancer cells (MCF-7) and human Sertoli cells (HSeC) were used for cell culture. MCF-7 and HSeC were exposed to BPA, BPS, BPF, BPZ, BPAF for 24 h. After that, the inhibitory effect of Bisphenols (IC50) was determined by measuring the absorbance. While BPF was the least cytotoxic alternative depending on the highest IC50 values in both cell lines, BPZ was found to be the most cytotoxic alternative in HSeC cell line. In the MCF-7 cell line, BPA and BPZ were found to have equally cytotoxic effects.