Comparison of different pretreatments in ethanol fermentation using corn cob hemicellulosic hydrolysate with Pichia stipitis and Candida shehatae

Eken-Saracoglu N., Arslan Y.

BIOTECHNOLOGY LETTERS, vol.22, no.10, pp.855-858, 2000 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 10
  • Publication Date: 2000
  • Doi Number: 10.1023/a:1005663313597
  • Title of Journal : BIOTECHNOLOGY LETTERS
  • Page Numbers: pp.855-858


A hemicellulosic hydrolysate was prepared with 0.3 M H2SO4 at 98 degrees C for 1 h. The total initial reducing sugar was maintained at 45 g l(-1) by synthetic xylose supplementation. The seven detoxification methods were employed including either the single addition of solid CaO (to pH 10 or 6) or its combinations with zeolite shaking. Over-liming gave the hydrolysate that was most completely fermented by Pichia stipitis and Candida shehatae at 30 degrees C, pH 6, among the tested methods.