Determining Genotoxic Effects Of Dicamba On Allium cepa using Comet Test in Tissue Culture


Özel Ç. A. , Avuloğlu Yılmaz E., Erikel E., Ünal F. , Mirici S. , Yüzbaşıoğlu D.

3rd International Conference on Engineering Technology and Applied Sciences (ICETAS), Skopje, Makedonya, 17 - 21 Temmuz 2018, ss.19

  • Basıldığı Şehir: Skopje
  • Basıldığı Ülke: Makedonya
  • Sayfa Sayıları: ss.19

Özet

Dicamba (3,6-dichloro-2-methoxybenzoic acid) is a benzoic acid derivative synthetic auxin that is
used in plant cell and tissue culture. It is generally applied as herbicide to kill perennial plants. There
are negative effects of plant growth regulators on environment due to their careless use, however,
very few studies report their negative effects on genetic material. It is possible to determine
genotoxicity using different test systems that measure damages to genetic material. Allium cepa is
used as a model plant in performing these tests including comet assay. This study investigated
possible genotoxic effects of dicamba on Allium cepa root meristems. Hence, bulbs were rooted on
agar solidified MS medium for 5 days. Thereafter, the bulbs were transferred to Murashige and
Skoog (MS) medium (control) or MS medium containing 0.67, 1.34, 2.01, 2.68, 3.35, 4.02 and 8.04
mg/L dicamba for 24 hours. All concentrations of dicamba had significant damaging effects on roots
that measured as comet tail intensity and tail moment compared to the control group. Almost all
concentrations of dicamba showed damaging effects on DNA.

Keywords: Dicamba, Allium Cepa, Comet Assay, Tissue Culture