Molecular characterization of a human group C rotavirus detected first in Turkey


Mitui M. T. , BOZDAYI G., DALGIÇ B., Bostanci I., Nishizono A., Ahmed K.

VIRUS GENES, vol.39, no.2, pp.157-164, 2009 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1007/s11262-009-0420-8
  • Journal Name: VIRUS GENES
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.157-164

Abstract

The present study was done to find out the prevalence of group B and C rotavirus infections in children with diarrhea presented at two major hospitals in Ankara, Turkey. Group B rotavirus was not found in any samples. One of 122 samples was positive for group C rotavirus. Phylogenetic analysis of genes for nonstructural protein NSP4, and structural proteins VP4, VP6, and VP7 confirmed the human origin of this strain. Similar to other human group C rotaviruses, one N-glycosylation site was predicted at amino acid residue 67 on the VP7 of strain GUP188. The genes of strain GUP188 were closely related to those of human group C rotavirus strain from the UK (Bristol) for NSP4, China (208 and Wu82) for VP4 and VP6, and from Colombia (Javeriana) for VP7, indicating that the Turkish group C rotavirus was unique and can serve as an additional reference strain for the molecular epidemiology of group C rotaviruses.