Non-intercalative, Deoxyribose Binding of Boric Acid to Calf Thymus DNA

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Ozdemir A., Gursacli R. T., Tekinay T.

BIOLOGICAL TRACE ELEMENT RESEARCH, cilt.158, sa.2, ss.268-274, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 158 Sayı: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s12011-014-9924-8
  • Dergi Adı: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.268-274
  • Anahtar Kelimeler: Calf thymus DNA, Boric acid, Spectroscopy, Bindingmode, FT-IR, NEUTRON-CAPTURE THERAPY, CANCER, STABILITY, DELIVERY, TUMORS
  • Gazi Üniversitesi Adresli: Evet

Özet

The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV-Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid-DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K (a)) of 9.54 x 10(4) M-1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations.