Non-intercalative, Deoxyribose Binding of Boric Acid to Calf Thymus DNA


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Ozdemir A., Gursacli R. T., Tekinay T.

BIOLOGICAL TRACE ELEMENT RESEARCH, vol.158, no.2, pp.268-274, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 158 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1007/s12011-014-9924-8
  • Journal Name: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.268-274
  • Keywords: Calf thymus DNA, Boric acid, Spectroscopy, Bindingmode, FT-IR, NEUTRON-CAPTURE THERAPY, CANCER, STABILITY, DELIVERY, TUMORS
  • Gazi University Affiliated: Yes

Abstract

The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV-Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid-DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K (a)) of 9.54 x 10(4) M-1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations.