Bioassay-guided isolation of kaempferol-3-O-β-d-galactoside with anti-inflammatory and antinociceptive activity from the aerial part of Calluna vulgaris L.

Orhan İ., Kiipeli E., Terzioglu S., Yesilada E.

Journal of Ethnopharmacology, vol.114, no.1, pp.32-37, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 114 Issue: 1
  • Publication Date: 2007
  • Doi Number: 10.1016/j.jep.2007.06.017
  • Journal Name: Journal of Ethnopharmacology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.32-37
  • Keywords: anti-inflammatory activity, antinociceptive activity, calluna vulgaris, ericaceae, heather, kaempferol-3-0-beta-d-galactoside, GLYCOSIDES, ERICACEAE, CYCLOOXYGENASE, PHYTOCHEMISTRY, FLAVONOIDS, INHIBITION, PLANTS
  • Gazi University Affiliated: Yes


Calluna vulgaris L. (Ericaceae) is used for the treatment of various inflammatory ailments in traditional medicines. In order to evaluate this ethnobotanical information, its anti-inflammatory and antinociceptive activities were studied using in vivo experimental models in mice. The ethanolic extract of the plant was first fractionated into five extracts; namely, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and water fractions. Among them, the EtOAc Fr. was found to be the most effective and was further subjected to bioassay-guided fractionation and isolation procedures. After successive column chromatography applications, on Sephadex LH-20 and silica gel, a component, which is responsible for the above-mentioned activities of this species of Turkish origin, was isolated and its structure was elucidated as kaempferol-3-O-β-d-galactoside, a common flavonol derivative by means of spectral techniques. © 2007 Elsevier Ireland Ltd. All rights reserved.