The aim of this study is to reveal chromosomal properties of endemic Seminemacheilus lendlii fish species. Metaphase plates were obtained from kidney cells. The diploid chromosome number was observed 2n=50; consisting of eight pairs of metacentric, 10 pairs of submetacentric and seven pairs of subtelocentric chromosomes. The fundamental number of chromosomal arms (FN) was found as 86. Positive C-bands were seen on the centromeric region for several chromosomes. Also, heterochromatic blocks were determined on the short and/or long arms of some chromosome pairs. Four nucleolus organizer regions (NORs) were observed on the silver stained metaphase plates. This study may contribute to cytogenetic data of Anatolian loaches.