Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes


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Celik M., ÜNAL F. , Yuzbasioglu D. , Yilmaz S., Aksoy H., Karaman S.

Cytotechnology, cilt.51, sa.2, ss.99-104, 2006 (SCI Expanded İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 51 Konu: 2
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1007/s10616-006-9015-6
  • Dergi Adı: Cytotechnology
  • Sayfa Sayıları: ss.99-104

Özet

In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10 -5 , 10 -4 , 10 -3 and 10 -2 μl ml -1 concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 μg ml -1 ) plus 10 -5 , 10 -4 , 10 -3 and 10 -2 μl ml -1 concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10 -2 μl ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10 -4 , 10 -3 and 10 -2 μl ml -1 concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control. © 2006 Springer Science+Business Media, Inc.