Development of a molecularly imprinted solid-phase extraction sorbent for the selective extraction of telmisartan from human urine


YILMAZ H., BASAN H.

JOURNAL OF SEPARATION SCIENCE, vol.38, no.8, pp.1433-1439, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 8
  • Publication Date: 2015
  • Doi Number: 10.1002/jssc.201401349
  • Journal Name: JOURNAL OF SEPARATION SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1433-1439
  • Keywords: Human urine, Molecularly imprinted polymer, Solid-phase extraction, Telmisartan, II RECEPTOR ANTAGONISTS, MASS-SPECTROMETRY METHOD, HUMAN PLASMA, MICROEXTRACTION, HPLC, IMMUNOASSAY, POLYMERS
  • Gazi University Affiliated: Yes

Abstract

A novel molecularly imprinted solid-phase extraction with spectrofluorimetry method has been developed for the selective extraction of telmisartan from human urine. Molecularly imprinted polymers were prepared by a noncovalent imprinting approach through UV-radical polymerization using telmisartan as a template molecule, 2-dimethylamino ethyl methacrylate as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, N,N-azobisisobutyronitrile as an initiator, chloroform as a porogen. Molecularly imprinted polymers and nonimprinted control polymer sorbents were dry-packed into solid-phase extraction cartridges, and eluates from cartridges were analyzed using a spectrofluorimeter. Limit of detection and limit of quantitation values were 11.0 and 36.0 ng/mL, respectively. A very high imprinting factor (16.1) was achieved and recovery values for the telmisartan spiked in human urine were in the range of 76.1-79.1%. In addition, relatively low within-day (0.14-1.6%) and between-day (0.11-1.31%) precision values were obtained. Valsartan was used to evaluate the selectivity of sorbent as well. As a result, a sensitive, selective, and simple molecularly imprinted solid-phase extraction with spectrofluorimetry method has been developed and successfully applied to the direct determination telmisartan in human urine.