Evaluation of the cytotoxic and genotoxic potential of lecithin/chitosan nanoparticles

Taner G., Yesiloz R., Vardar D. O., Senyigit T., Ozer O., Degen G. H., ...Daha Fazla

JOURNAL OF NANOPARTICLE RESEARCH, cilt.16, sa.2, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s11051-013-2220-2
  • Dergi Adı: JOURNAL OF NANOPARTICLE RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Lecithin/chitosan nanoparticles, Neutral red uptake assay, Cytokinesis-blocked micronucleus assay, Comet assay, Nanomedicine, COMET ASSAY, DNA-DAMAGE, CHITOSAN NANOPARTICLES, IN-VITRO, LYMPHOCYTES, MICRONUCLEI, DELIVERY, BLOOD
  • Gazi Üniversitesi Adresli: Evet

Özet

Nanoparticles-based drug targeting delivery systems have been introduced in the treatment for various diseases because of their effective properties, although there have been conflicting results on the toxicity of nanoparticles. In the present study, the aim was to evaluate the cytotoxicity and the genotoxicity of different concentrations of lecithin/chitosan nanoparticles with and without clobetasol-17-propionate (CP) by neutral red uptake (NRU) cytotoxicity assay and single cell gel electrophoresis (Comet) and cytokinesis-blocked micronucleus assays. The IC50 values of lecithin/chitosan nanoparticles with/without CP were found as 1.9 and 1.8 %, respectively, in the NRU cytotoxicity test. High concentrations of lecithin/chitosan nanoparticles induced DNA damage in human lymphocytes as evaluated by comet assay. The micronucleus frequency was increased by the lecithin/chitosan treatment in a dose-dependent manner. Also at the two highest concentrations, a significant increase in micronucleus formation was observed. Lecithin/chitosan nanoparticles with CP did not increase the frequency of micronucleus and also did not induce additional DNA damage when compared with lecithin/chitosan nanoparticles without CP; therefore, CP itself has not found to be genotoxic at the studied concentration.