Akademik Veri Yönetim Sistemi
JOURNAL OF THE INDIAN CHEMICAL SOCIETY, cilt.103, sa.7, ss.1-9, 2026 (SCI-Expanded, Scopus)
Rapid, economical determination of glucose levels in food samples with high sensitivity and precision is essential for food quality processes. Enzyme biosensors are widely used bioanalytical devices that offer fast response times, high selectivity, and high sensitivity to analytes. The working electrodes of enzyme biosensors are modified with metallic nanoparticles to enhance performance. Metallic nanoparticles used for electrode modification are produced via green synthesis techniques, enabling the design of eco-friendly, cost-effective biosensors. In this study, coffee grounds, a common household waste, were used to green synthesize of silver nanoparticles. The obtained Turkish coffee grounds-based silver nanoparticles (TC-AgNPs) were characterized and used to modify a carbon paste electrode. Glucose oxidase was immobilized on the surface of the modified carbon paste electrode using a cross-linking technique, and glucose determination was based on the oxidation of H2O2 released by the reaction at an Ag/AgCl electrode at +0.5 V. The linear operating range of the designed biosensor was determined to be 0.1-0.5 nM and 0.1-1.0 μM. It was determined that after 12 repetitions, the biosensor retained 83.85% of its initial activity. It could be concluded that a new eco-friendly and economical glucose biosensor was designed with low interference, high selectivity, good reproducibility, and a long shelf life.