In this study, enzyme inhibitory and antioxidant activity of the ethanol extracts of Centella asiatica (L.) Urban from Turkey and India was examined and the results were compared to those of the standardized extract of the plant obtained from China. Inhibitory activity of these three extracts was screened against four enzymes; acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase (TYRO), and lipoxygenase (LOX) using ELISA microplate reader. Antioxidant activity of the extracts was determined in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH), metal-chelation, and ferric-reducing antioxidant power (FRAP) assays. The extracts showed a notable inhibition against BChE and TYRO, while the extracts from Turkey and India displayed lower activity in the antioxidant assays. HPLC analysis indicated that the Turkish plant sample was found to be richest in p-hydroxy-benzoic acid, whilst the Indian sample contained chlorogenic acid in the most abundant amount. alpha-Copaene was the dominant compound in the essential oil of the plant sample from Turkey. (c) 2013 Elsevier B.V. All rights reserved.