BIOTECHNIC & HISTOCHEMISTRY, cilt.74, sa.2, ss.57-63, 1999 (SCI-Expanded)
The fetal skeleton double staining method is used to reveal developmental abnormalities in the skeletal system. We used alizarin red S and alcian blue successfully with microwave irradiation for skeletal double staining. The fixation time was reduced from 4-7 days to 2-2.5 min and the staining time was reduced from 4 days to 23 min.