Double staining of skeleton using microwave irradiation


Ilgaz C., Kocabiyik H., Erdogan D., Ozogul C., Peker T. V.

BIOTECHNIC & HISTOCHEMISTRY, vol.74, no.2, pp.57-63, 1999 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 2
  • Publication Date: 1999
  • Doi Number: 10.3109/10520299909066479
  • Title of Journal : BIOTECHNIC & HISTOCHEMISTRY
  • Page Numbers: pp.57-63

Abstract

The fetal skeleton double staining method is used to reveal developmental abnormalities in the skeletal system. We used alizarin red S and alcian blue successfully with microwave irradiation for skeletal double staining. The fixation time was reduced from 4-7 days to 2-2.5 min and the staining time was reduced from 4 days to 23 min.