Sequence-specific label-free nucleic acid biosensor for the detection of the hepatitis C virus genotype 1a using a disposable pencil graphite electrode


DÖNMEZ S., ARSLAN F., ARSLAN H.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.44, no.3, pp.912-917, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.3109/21691401.2014.998831
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.912-917
  • Keywords: hepatitis C virus, inosine, nucleic acid biosensor, pencil graphite electrode, square wave voltammetry, FREE ELECTROCHEMICAL DNA, TARGET RECYCLING STRATEGY, MYCOBACTERIUM-TUBERCULOSIS, HYBRIDIZATION BIOSENSOR, INDICATOR, SENSOR, CARBON, ADENINE, GUANINE, CHAIN
  • Gazi University Affiliated: Yes

Abstract

In this paper, we demonstrate a simple, sensitive, inexpensive, disposable and label-free electrochemical nucleic acid biosensor for the detection of the hepatitis C virus genotype 1a (HCV1a). The nucleic acid biosensor was designed with the amino-linked inosine-substituted 20-mer probes, which were immobilized onto a disposable pencil graphite electrode (PGE) by covalent linking. The proposed nucleic acid biosensor was linear in the range of 0.05 and 0.75 M, exhibiting a limit of detection of 54.9 nM. The single-stranded synthetic PCR product analogs of HCV1a were also detected with satisfactory results under optimal conditions, showing the potential application of this biosensor.