Recombinant adiponectin inhibits inflammation processes via NF-kB pathway in acute pancreatitis

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Dikmen K., Bostanci H., Gobut H., Yavuz A., Alper M., Kerem M.

BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, sa.10, ss.619-624, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2018
  • Doi Numarası: 10.4149/bll_2018_110
  • Dergi Adı: BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.619-624
  • Anahtar Kelimeler: acute pancreatitis, recombinant adiponectin, cytokines, nuclear factor kappa B, KAPPA-B ACTIVATION, NUCLEAR FACTOR-KAPPAB, NECROTIZING PANCREATITIS, OXIDATIVE STRESS, PROTECTIVE ROLE, EXPRESSION, INJURY, MODEL, MICE, IL-6
  • Gazi Üniversitesi Adresli: Evet

Özet

PURPOSE: Adiponectin is a protein stemming from adipose tissue and having strong anti-inflammatory properties. We aimed to assess the damage diminishing effects of recombinant adiponectin (rAD) through NF-kB in the experimental acute pancreatitis (AP) model. MATERIALS AND METHODS: Acute pancreatitis was created by applying 50 mu g/kg dose of intraperitoneal cerulean. The rats were randomised and divided into 3 groups as control, AP and rAD groups. Moreover, the rats in each group were divided into two sub-groups as 24th and 48th hour subgroups. rAD was injected in the study group intraperitoneally. Tissue and blood samples were taken after 24 and 48 hours. Histopathological assessment and NF-kB activity were investigated in pancreatic tissue. RESULTS: Serum TNF-a, IL-1b and IL-6 levels were found to be statistically significant in the AP group compared to the rAD group in the 24th and 48th hour (p < 0.05). Similarly, NF-kB activity was also found to be significant in the AP group both in the 24th and 48th hour (p < 0.05). There were significant differences in the AP and the rAD groups histopathologically in terms of edema, inflammation, vacuolisation and necrosis (p < 0.001). CONCLUSION: rAD has significantly reduced NF-kB activity, cytokine levels and tissue damage.