There is a positive effect of exogen vascular endothelial growth factor (VEGF) application to diabetic wound. This study aims to research the effects of topical VEGF administration on oxidative parameters in rat serum. In this study, 36 female wistar albino rats (weight: 200-250 g) were used. All rats got diabetic with streptozotocin. Dorsalateral excisional wounds (4 cm lengthy) were made on the two sides of medulla spinalis on the rats and they were randomly divided into 3 groups: untreated groups (n=12), chitosan treated (n=12) and chitosan+ VEGF (n=12) (7 ng/ml). In chitosan treated groups, wounds treated topically with equal amount blank chitosan gel. After this administration on the 3 th and 7 th days of wound, animals were sacrified. NOx, TBARS and RSH levels were measured spectrophotometrically in serum. Results were expressed as mean +/- standart deviation. Mean differences were compared by Anova Variance Analysis (p<0,05).