Formation of PAH-DNA adducts after in vivo and vitro exposure of rats and lung cells to different commercial carbon blacks

Borm P., ÇAKMAK G., Jermann E., Weishaupt C., Kempers P., van Schooten F., ...Daha Fazla

TOXICOLOGY AND APPLIED PHARMACOLOGY, cilt.205, sa.2, ss.157-167, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 205 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.taap.2004.10.020
  • Dergi Adı: TOXICOLOGY AND APPLIED PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.157-167
  • Anahtar Kelimeler: carbon black, bioavailability, PAHs, DNA adducts, lung, DIESEL EXHAUST, INHALED PARTICLES, CANCER, DAMAGE, GENOTOXICITY, PARTICULATE, INHALATION, CARCINOGEN, PART
  • Gazi Üniversitesi Adresli: Evet

Özet

Objective: The current study was designed to test the possible release and bioavailability of polycyclic aromatic hydrocarbons (PAHs) from a set of commercial carbon blacks (CBs) as well as the ability of these PAHs to form bulky DNA adducts. Methods: In four commercial CBs (Printex 90, Sterling V, N330, Lampblack 101), leaching of PAH was examined through (1) release of parent PAHs in saline with or without surfactant, and (2) PAH adducts in lung epithelial cells (A549) or in rat lungs after exposure to two CBs (Printex 90, Sterling V) for 13 weeks (50 mg/m(3)). In vitro experiments were done with original and extracted particles, as well as organic extracts of CB in DMSO. As positive controls, B[a]P (0.03 mu M) and a mixture of 16 PAHs (0.1 mu M) were used. Results: No leaching of PAHs was measured in saline or surfactant-containing saline. In vitro incubations with CB particles (30-300 mu g/cm(2)) revealed no adduct spots except for Sterling V. However, the spot was not concentration dependent and remains unidentified. Lung DNA from rats after inhalation of Printex 90 or Sterling V showed no spots related to PAH-DNA adduct formation compared to sham-exposed rats. Conclusion: The results suggest that PAHs are very tightly bound to these CBs. Only using organic extracts or particles of low-surface Sterling V, with high PAH content, PAHs may become available to form PAH-DNA adducts. However, the in vitro conditions showing this effect will not be encountered in vivo and renders this mechanism in particle-induced lung cancer at in vivo exposures highly unlikely. (c) 2004 Elsevier Inc. All rights reserved.