An amperometric biosensor for uric acid determination prepared from uricase immobilized in polyaniline-polypyrrole film

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Arslan F.

SENSORS, vol.8, no.9, pp.5492-5500, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 9
  • Publication Date: 2008
  • Doi Number: 10.3390/s8095492
  • Journal Name: SENSORS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.5492-5500
  • Keywords: uric acid, uricase, biosensor, polypyrrole, polyaniline, ENZYME ELECTRODE, OXIDASE
  • Gazi University Affiliated: Yes


A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5x10(-6)-8.5x10(-5) M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 degrees C, respectively. The stability and reproducibility of the enzyme electrode have been also studied.