Akademik Veri Yönetim Sistemi
VI. International Scientific and Vocational Studies Congress – Science and Health (BILMES SH 2021), Tokat, Türkiye, 23 Aralık 2021, ss.7-8
Magnetic resonance imaging (MRI) is an important
imaging method that provides detailed cross-sectional and three-dimensional
anatomical images of tissues and organs and is used in the diagnosis and
monitoring of various diseases. In the cases of insufficient resolution and low
contrast in MR images, contrast agents are administered to patients. Contrast
agents are diagnostic pharmaceutical materials comprising paramagnetic or
superparamagnetic metal ions affecting the MR-signal characteristics of
tissues, enhancing the image aspect of MRI and the appearance of the internal
structure of the body by making clear the
density difference between tissues. Since gadolinium (III) is the most widely
used heavy metal in MRI contrast agents due to its high magnetic moment and
stable structure, gadolinium-based contrast agents (GBCAs) have been commonly
used in daily practice. Such widespread use of contrast agents has brought with
it some health concerns. Therefore, this study aimed to examine genotoxic
effects of Gadobutrol, one of the most used GBCAs in MRI, by Sister Chromatid
Exchange (SCE) assay. Lymphocytes taken from three healthy donors were treated
with 7,000, 14,000, 28,000, 56,000, and 112,000 μg/mL concentrations of
Gadobutrol for 24 and 48 hours. Distilled water and Mitomycin-C were used as the negative and positive control, respectively. After 24 and 48-hour treatments of gadobutrol, all concentrations were found to increase SCEs in human lymphocytes in a
dose-dependent manner compared to the negative control. While only 112,000
μg/mL concentration of Gadobutrol was found to be significant for 24-hour
treatments, both 56,000 and 112,000 μg/mL were significant for 48-hour
treatments. A dose-dependent decrease in the mitotic index (MI)
was observed in both the 24-hour and 48-hour treatments compared to the
negative control, with concentrations of 56,000 and 112,000 μg/mL being statistically significant. Gadobutrol did
not cause a significant difference in the replication index. These results
display that Gadobutrol leads to inhibition of mitotic division and induces
SCEs at higher concentrations in human lymphocytes in vitro. Besides, other in
vitro and in vivo test systems
should be carried out with this contrast agent to evaluate its genotoxic
potential more clearly.