This study aims to determine the amount of organochlorine pesticides (OCPs), especially DDT, its metabolites, and BHC isomers, in human milk samples obtained from Afyon city. For this purpose, OCP contamination levels were determined in 80 human milk samples from healthy donors living in Afyon for at least 5 years by GC-ECD analysis with aldrin as internal standard. Alpha-benzenehexachloride (0.027±0.05), beta-BHC (0.285±0.286) gamma-BHC (0.014±0.055), HCB (0.073±0.087), heptachlor epoxide (0.061±0.061), p,p′-DDE (2.098±1.379), and p,p′-DDT (0.111±0.294), expressed on a fat basis (ppm), could be detected. The results have been discussed in terms of regions and OCPs. © by PSP.